Innovative Microchip Solutions
Advanced technologies for cell positioning, impedance monitoring, and personalized therapy in biochemistry and diagnostics.
Innovative Microelectrode Solutions
We specialize in semiconductor manufacturing, bio-sensing, and personalized therapy solutions through advanced technologies.
Programmable Nanochip Technology
Our 3D nanochips significantly enhance cell viability and the efficiency of transfection and biodetection. The nanoelectrodes are separated by a gap of 70 nm, with each electrode having a tip diameter of 40 nm. When voltage is applied between the nanoelectrodes, it generates a strong electric field that electroporates multiple nanolocalized areas of the target single-cell membrane. This process gently transports biomolecules into the cells through pressurized pump flow without compromising cell viability.
This platform successfully delivers target and plasmids to various cell types by adjusting the field strength, pulse duration, and pulse number. This innovative method allows us to analyze the delivery of different biomolecules into single living cells with exceptionally high transfection efficiency (>96% for CL1-0 cells) and high cell viability (~98%). These capabilities are particularly advantageous for cell therapy and monitoring applications.
Single-Cell Personalized Approach
Efficiently transfect multiple cell lines for targeted therapy and diagnostics applications.
Individualized electroporation of single cells utilizes micro-electrode array for real-time impedance monitoring.
Microelectrode chips facilitate the precise and efficient delivery of biomolecules like DNA, RNA, and proteins into cells. By optimizing parameters such as voltage, pulse number, pulse width, and the choice of buffer and reagents, these chips enhance the transfection efficiency, especially for cells that are challenging to transfect. A minimal amount of DNA can be used, which helps in reducing cell death associated with cytotoxicity. Electroporation is versatile and applicable across various cell types, including bacteria, yeast, plants, and animals, making it a valuable technique in cellular research and biotechnology.